MOLECULAR STUDY GENOTYPE HLA-DQB1 (high resolution), whole blood

New Test in NOÛS Catalog

Test Code: 5263

Sample:	Whole blood EDTA (10 ml)
Conservation:	Refrigerated
Method:	PCR-SSO
Set Up Days:	Daily
Delivery term:	15 days
Information:
The major histocompatibility complex (MHC), is a family of genes constitute the so-called HLA (for human leukocyte antigen) system; they are essential in the body's immune defense against pathogens, and on the other hand, constitute the main barrier to organ transplantation and stem cell. Its discovery has enabled medicine to make a qualitative leap on the chances for success of a transplant, opening a promising path, whose major trouble was the organ rejection. It could also be discovered connection between certain HLA profiles and a higher frequency of autoimmune diseases such as systemic lupus erythematosus, myasthenia gravis, Sjögren's syndrome, or other like ankylosing spondylitis and celiac disease, etc. Until recently, serological techniques have used and they have based on cytotoxicity (cell lysis mediated by specific antibodies in the presence of complement); the technique is manual, cumbersome and its interpretation is subjective. The development in molecular biology techniques has enabled the use of more precise techniques, with higher reducibility and resolution and higher automation. PCR-SSOP (polymerase chain reaction-sequence specific oligonucleotide probe) is the most commonly used method due to its and in cases where higher resolution is required to allelic level, the PCR-SSP technique is used (polymerase chain reaction-sequence specific primers). For this reason, Labco NOÛS has decided to support the integration of these new techniques instead of the traditional methods.

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