INTERFERON GAMMA (QUANTIFERON TB), PLASMA

 Sample collection Modification

Test code: 3147

Effective update from 16/01/2017

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NEW

Three tubes:   - Grey tube (control)   - Red tube (TB antigens)  - Purple tube (mitogen)

Sample tubes: Test-specific tubes that contain test- specific reagents. 4 tubes are needed:       Quantiferon TB1: green cap      Quantiferon TB2: yellow cap      Quantiferon Nil: grey cap      Quantiferon Mitogen: purple cap Tubes must be kept at room temperature (between 4 and 25ºC). Gently mix the tube content before blood drawing.  1- Collect 1mL of blood by venipuncture directly into each of the 4 blood collection tubes. The black mark on the side of the tubes indicates the 1mL fill volume. Ensure that the correct volume is drawn 2-  Immediately after filling tubes, shake them ten (10) times just firmly enough to ensure the entire inner surface of the tube is coated with blood to solubilize the tube content 3-  Mark or label tubes taking care of not impairing the content visibility 4-  Incubate tubes at 37ºC +/- 1, for 16-24h  5-  Centrifuge at 2.000-3.000 g for 15 min 6-  Gently separate plasma       7-  Test samples or keep plasma (up to 28 days at 2-8ºC or longer periods at temperatures equal or under -20ºC)