Test code: 4345
Effective update from 26/10/2018
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Not detected
Methodology used:
Biological sample's DNA extracted by using automatic system MagNA Pure (Roche Diagnostic).
Detection of Cytomegalovirus by PCR and subsequent amplification of regions UL97 and UL54, according to Mendez et al. protocol and Boivin et al. (J. M. Virology, 2005).
Two-way sequencing of the amplification products obtained from the regions UL97 (Aas 462-664) and UL54 (Aas 363-993) by automatic sequencer ABI 3130XL from Applied Biosystem. Analysis of DNA sequences Polymerase and CMV phosphotransferase using SeqScape v2.5 software and the Mutation Analyzer bioinformatics tool.
Amplification of a FV fragment as internal control for extraction and amplification.
The results collected in this report only refer to the sample tested.
Bibliography:
1. Chou, S., Ercolani, R. J. & Vanarsdall, A. L. Differentiated Levels of Ganciclovir Resistance Conferred
by Mutations at Codons 591 to 603 of the Cytomegalovirus UL97 Kinase Gene. J. Clin. Microbiol. 55,
2098–2104 (2017).
| Not detected |
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