Friday, October 26, 2018

Leptospira spp, DNA, biological sample

Reference values Modification

Test code: 3680

Effective update from 26/10/2018


DNA was extracted from blood using automatic system MagNA Pure (Roche Diagnostic).

Amplification by nested-PCR was performed with specific and generic primers for lipL32 gene of Leptospira. The amplified fragment (316 bp in first amplification and 186 pb in the second) was checked by agarose gel with ethidium bromide staining.
Amplification of the Factor V gene has been carried out as an extracion and aplification control.

The results of this test are only for the tested sample. 


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