Test code: 3467
Mnemonic code: B5701
Effective update from 06/11/2023
Effective update from 06/11/2023
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Negative METHODOLOGY: DNA extraction from peripheral blood. Amplification by RT-PCR (real-time polymerase chain reaction) using specific primers to identify alleles HLA-B*5701. HGB gene hasbeen analysed as internal control. Detection was doing by SYBR Green stain and melting curve. NOTE: HLA-B*5701 allele is associated to a hipersensitivity reaction to anti-HIV Abacavir drug. Version of Test: 3 | Negative Technique: DNA extraction from whole blood and subsequent amplification by real-time PCR (CE-IVD commercial method) of the alleles of the HLA-B*57:01 group associated with a hypersensitivity reaction to abacavir, and of a fragment of the HBB (ß-globin) gene used as an internal positive control. Version of Test: 4 |
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