HLA-B5701 genotype (susceptibility to Abacavir), total blood

Reference values Modification

Test code: 3467

Mnemonic code: B5701

Effective update from 06/11/2023

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Negative             METHODOLOGY: DNA extraction from peripheral blood. Amplification by RT-PCR (real-time polymerase chain reaction) using specific primers to identify alleles HLA-B*5701. HGB gene hasbeen analysed as internal control. Detection was doing by SYBR Green stain and melting curve.       NOTE: HLA-B*5701 allele is associated to a hipersensitivity reaction to anti-HIV Abacavir drug.                   Version of Test: 3

Negative Technique: DNA extraction from whole blood and subsequent amplification by real-time PCR (CE-IVD commercial method) of the alleles of the HLA-B*57:01 group associated with a hypersensitivity reaction to abacavir, and of a fragment of the HBB (ß-globin) gene used as an internal positive control.                              Version of Test: 4

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