Thursday, October 26, 2023

HLA-B5701 genotype (susceptibility to Abacavir), total blood

Reference values Modification

Test code: 3467
Mnemonic code: B5701

Effective update from 06/11/2023


      METHODOLOGY: DNA extraction from peripheral blood. Amplification by RT-PCR (real-time polymerase chain reaction) using specific primers to identify alleles HLA-B*5701. HGB gene hasbeen analysed as internal control. Detection was doing by SYBR Green stain and melting curve.
      NOTE: HLA-B*5701 allele is associated to a hipersensitivity reaction to anti-HIV Abacavir drug.
      Version of Test: 3


Technique: DNA extraction from whole blood and subsequent amplification by real-time PCR (CE-IVD commercial method) of the alleles of the HLA-B*57:01 group associated with a hypersensitivity reaction to abacavir, and of a fragment of the HBB (ß-globin) gene used as an internal positive control.         
Version of Test: 4

   Find the record of the test by clicking here

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