Rubeola virus (RUBV) RNA, biological sample

Reference values and Delivery Time Modification

Test code: 3009

Effective update from 07/05/2018

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Not detected.             * This technique has sensitivity 99% and       specificity of 98%.             Méthodology used:       RNA extraction from the received sample was performed by means of the automated system MagNA Pure (Roche Diagnostic). Following, amplification (nested-PCR) was performed with specific primers targeting a fragment of the glycoprotein E1 from RUBV following Mosquera's et al. (Journal Clinical Microbiology, Vol40 nº 1; 2002) protocol. The detection of the amplified fragment (289 pb) was done by visualization in an agarose gel after staining with ethidium bromide.       Amplification of a FV fragment as internal control for extraction and amplification.             The results of this report refer only to the tested sample.                   Delivery term: 10 days

Not detected.             * This technique has sensitivity from 200 to 300 copies/mL and specificity of 100 %.                                                                                                                 Delivery term: 4 days

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