Friday, May 11, 2018

Rubeola virus (RUBV) RNA, biological sample

Reference values and Delivery Time Modification

Test code: 3009

Effective update from 07/05/2018


      Not detected.
      * This technique has sensitivity 99% and
      specificity of 98%.
      Méthodology used:
      RNA extraction from the received sample was performed by means of the automated system MagNA Pure (Roche Diagnostic). Following, amplification (nested-PCR) was performed with specific primers targeting a fragment of the glycoprotein E1 from RUBV following Mosquera's et al. (Journal Clinical Microbiology, Vol40 nº 1; 2002) protocol. The detection of the amplified fragment (289 pb) was done by visualization in an agarose gel after staining with ethidium bromide.
      Amplification of a FV fragment as internal control for extraction and amplification.
      The results of this report refer only to the tested sample.
      Delivery term: 10 days

     Not detected.
      * This technique has sensitivity from 200 to 300 copies/mL and specificity of 100 %.      

Delivery term: 4 days

   Find the record of the test by clicking here

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