Test code: 3009
Effective update from 07/05/2018
* This technique has sensitivity 99% and
specificity of 98%.
RNA extraction from the received sample was performed by means of the automated system MagNA Pure (Roche Diagnostic). Following, amplification (nested-PCR) was performed with specific primers targeting a fragment of the glycoprotein E1 from RUBV following Mosquera's et al. (Journal Clinical Microbiology, Vol40 nº 1; 2002) protocol. The detection of the amplified fragment (289 pb) was done by visualization in an agarose gel after staining with ethidium bromide.
Amplification of a FV fragment as internal control for extraction and amplification.
The results of this report refer only to the tested sample.
Delivery term: 10 days
* This technique has sensitivity from 200 to 300 copies/mL and specificity of 100 %.
Delivery term: 4 days
Find the record of the test by clicking here