Conditions for the sample’s obtaining and transport for the Special Coagulation Test

The following list summarizes conditions for a proper treatment before the sample’s analysis for the next special coagulation laboratory tests:

             •       Protein C and S, activity and antigen

•       Functional and antigenic coaguation factors

•       Lupus anticoagulant

•       Plasminogen

•       Activated Protein C Resistance

•       C1-esterase inhibitor

•       Plasminogen Activator Inhibitor

The patient must be fasting for at least 8 hours to avoid possible interference by lipemia of the sample (rejection criteria).

Sampling should be done in citrate tube (references BD363097 or BD363079). Make up to the mark perfectly, and homogenize gently by inversion 4 to 5 times. Centrifuge immediately after extraction, at room temperature (15 to 25 ° C), for at least 15 minutes at 1500 g (or 12 minutes at 2000 g). Separate the plasma in aliquot tube (reference tube LT0032 and reference plug 014794). Immediately freeze the plasma at -20 ° C. Send it and keep it frozen until processing. Minimum plasma volume 700 μL and optimal volume of 1000 μL.

If it is possible, and to avoid incidents in the sample by thawing-freezing cycles, send an aliquot for every determination to be made.

Other rejection criteria are hemolysis and permanence at room temperature over 2 hours.

Thanks in advance for your collaboration, we remain at your disposal for any specific consultation.

In the following list the affected tests are detailed:

2135 - PROTEIN C (ACTIVITY), PLASMA
2136 - PROTEIN S (ACTIVITY), PLASMA
3223 - PROTEIN C (ANTIGEN), PLASMA
3224 - PROTEIN S (ANTIGEN), PLASMA
2682 - FACTOR II, PLASMA
2675 - FACTOR V, PLASMA2562 - FACTOR VII, PLASMA
2660 - FACTOR VIII, PLASMA
2741 - FACTOR IX, PLASMA
2683 - FACTOR X, PLASMA
2731 - FACTOR XI, PLASMA
2676 - FACTOR XII, PLASMA
2903 - FACTOR XIII, PLASMA
2904 - FACTOR VIII  - ANTIGEN, PLASMA
2690 - FACTOR VON WILLEBRAND ANTIGEN, PLASMA
5583 - LUPUS ANTICOAGULANT (RUSSELL), PLASMA
2188 - PLASMINOGEN CONCENTRATION, PLASMA
2780 - PLASMINOGEN ACTIVITY, PLASMA
3084 - APC RESISTANCE (ACTIVATED PROTEIN C RESISTANCE), PLASMA
880   - C1-ESTERASE INHIBITOR (ACTIVITY), PLASMA
3143 - INHIBITOR t-PA (PAI-1) ANTIGEN, PLASMA
1194 - Plasmingen Inhibitor activator Antigen, plasma