SYNLAB En: Karyotype, total blood, amniotic fluid, haematological, tissue, total cord blood, fibroblasts, culture, chorionic villus (culture)

Reference values Modification

Test code: 684, 907, 1106, 2260, 2533, 5446, 3474

Nemonic code: Does not apply

Effective update from 03/27/2023

Test Code: 684

Karyotype, total blood

Previous References Values

New References Values

Technique: Cell culture for 48-72 hours stimulated with PHA (phytohemagglutinin). G-Banded Chromosomal Analysis (2020). Resolution level: 400-550 bands. Minimum number of metaphases studied: 15.
Limitations: This result does not include chromosomal findings interpreted as normal variants (European Cytogeneticists Association (ECA) http://www.eca.eu/EN/GUIDELINES.html) and is subject to the limitations of this type of analysis. studies, such as, among others, the presence of certain mosaic situations, small chromosomal abnormalities and discrepancies between tissues.

4. Methodology
             Cell culture (48h and 72h) stimulated with PHA (phytohemaglutinin). Identification by G bands (ISCN 2020). Resolution: 400-550 bands. Minimum number of metaphases analyzed: 15.

5. Limitations and recommendations
             - The present result does not include the chromosomal findings interpreted as variants of normality (European Cytogeneticists Association (ECA) http://www.eca.eu/EN/GUIDELINES.html) and is subject to the limitations of this type of studies, such as, among others, the presence of certain situations in mosaic, chromosomal cryptic or small size anomalies and discrepancies between tissues.
             - This report must be accompanied by the corresponding karyotype image and must be interpreted within the clinical context of the patient and according to medical criteria.

Test Code: 907

Karyotype, amniotic fluid

Previous References Values

New References Values

Technique: Cell culture. Analysis of two independent cultures. G-Banded Chromosomal Analysis (2020). Resolution level: 400-550 bands. Minimum number of metaphases studied: 20.
Limitations: This result does not include the findings interpreted as variants of normality (European Cytogeneticists Association (ECA) http://www.eca.eu/EN/GUIDELINES.html) and is subject to the limitations of this type of study. such as, among others, the presence of certain mosaic situations, small chromosomal abnormalities, discrepancies between tissues and contamination with maternal cells.
This report must be accompanied by the image of the corresponding karyotype and must be interpreted within the clinical context of the patient and according to medical criteria.

4. Methodology
             Cell culture. Identification by G bands (ISCN 2020) from two independent cultures. Resolution: 400-550 bands. Minimum number of metaphases analyzed: 20.

5. Limitations and recommendations
             - The present result does not include the chromosomal findings interpreted as variants of normality (European Cytogeneticists Association (ECA) http://www.eca.eu/EN/GUIDELINES.html) and is subject to the limitations of this type of studies, such as, among others, the presence of certain situations in mosaic, chromosomal cryptic or small size anomalies, discrepancies between tissues and maternal contamination.
             - This report must be accompanied by the corresponding karyotype image and must be interpreted within the clinical context of the patient and according to medical criteria.

Test Code: 1016

Karyotype haematological

Previous References Values

New References Values

Technique: Cell culture (24 hours without stimulation and/or 72 hours stimulated with TPA (tetradecanoylphorbol acetate) or PHA (phytohemagglutinin), depending on diagnostic orientation). G-Banded Chromosomal Analysis (2020). Resolution level: 400-550 bands. Minimum number of metaphases studied: 20.
Limitations: This result does not include the findings interpreted as variants of normality (European Cytogeneticists Association (ECA) http://www.eca.eu/EN/GUIDELINES.html) and is subject to the limitations of this type of study. , mainly the absence of growth of pathological cells, the possible non-detection of some mosaics and small structural alterations.
This report must be accompanied by the image of the corresponding karyotype and must be interpreted within the clinical context of the patient and according to medical criteria.

             4. Methodology                                                                                             Cell culture, with or without stimulation with TPA (tetradecanoylphorbol acetate) or PHA (phytohemagglutinin), depending on diagnostic orientation*. Identification by G bands (ISCN 2020) in two independent cultures. Resolution: 300-400 bands. Minimum number of metaphases analyzed: 20

             *1) Myeloproliferative neoplasia, Myelodysplastic syndrome, Acute leukemia, not indicated: direct cultures and 24 hours without mitogen. 2) Multiple myeloma: direct cultures and 72 hours without mitogen. 3)Chronic Lymphoproliferative Syndrome of B cells, Chronic Lymphoid Leukemia: direct cultures and 72 hours stimulated with TPA. 4) Chronic Lymphoproliferative Syndrome of T cells: direct cultures and 72 hours stipulated with PHA.

5. Limitations and recommendations
             - This result is issued following the criteria of the European Cytogeneticists Association (ECA),http//www.e-c-a.eu/EN/GUIDELINES.html).
             - This study does not include chromosomal findings interpreted as normal variants and is subject to the limitations of this type of study, mainly the absence of growth of pathological cells, the possible non-detection of some mosaics and small structural alterations.
             - The guidelines indicate that the conventional karyotype is not the most recommended analysis in cases of MM, although it can be performed to complete the study if there is a sufficient sample (Multiple Myeloma Guideline of the Spanish Group, 2021).
             - This report must be accompanied by the corresponding karyotype image(s) and must be interpreted within the clinical context of the patient and according to medical criteria.

Test Code: 2260

Karyotype, tissue

Previous References Values

New References Values

Technique: Cell culture. G-Banded Chromosomal Analysis (2020). Resolution level: 400-550 bands. Minimum number of metaphases studied: 15.
Limitations: This result does not include the findings interpreted as variants of normality (European Cytogeneticists Association (ECA) http://www.eca.eu/EN/GUIDELINES.html) and is subject to the limitations of this type of study. such as, among others, the presence of certain mosaic situations, small chromosomal abnormalities, discrepancies between tissues and contamination with maternal cells.
This report must be accompanied by the image of the corresponding karyotype and must be interpreted within the clinical context of the patient and according to medical criteria.

4. Methodology
             Cell culture. Identification by G bands (ISCN 2020). Resolution: 400-550 bands. Minimum number of metaphases analyzed: 15.

5. Limitations and recommendations
             - The present result does not include the chromosomal findings interpreted as variants of normality (European
             Cytogeneticists Association (ECA) http://www.eca.eu/EN/GUIDELINES.html) and is subject to the limitations of this type of studies, such as, among others, the presence of certain situations in mosaic, chromosomal cryptic or small size anomalies, discrepancies between tissues and maternal contamination.
             - This report must be accompanied by the corresponding karyotype image(s) and must be interpreted within the clinical context of the patient and according to medical criteria.

Test Code: 2533

Karyotype, total cord blood

Previous References Values

New References Values

Technique: Cell culture for 48-72 hours stimulated with PHA (phytohemagglutinin). G-Banded Chromosomal Analysis (2020). Resolution level: 400-550 bands. Minimum number of metaphases studied: 15.
Limitations: This result does not include chromosomal findings interpreted as normal variants (European Cytogeneticists Association (ECA) http://www.eca.eu/EN/GUIDELINES.html) and is subject to the limitations of this type of analysis. studies, such as, among others, the presence of certain mosaic situations, small chromosomal abnormalities and discrepancies between tissues.
This report must be accompanied by the image of the corresponding karyotype and must be interpreted within the clinical context of the patient and according to medical criteria.

4. Methodology
           Cell culture (48h and 72h) stimulated with PHA (phytohemaglutinin). Identification by G bands (ISCN 2020). Resolution: 400-550 bands. Minimum number of metaphases analyzed: 15.

             5. Limitations and recommendations
             - The present result does not include the chromosomal findings interpreted as variants of normality (European Cytogeneticists Association (ECA) http://www.eca.eu/EN/GUIDELINES.html) and is subject to the limitations of this type of studies, such as, among others, the presence of certain situations in mosaic, chromosomal cryptic or small size anomalies, discrepancies between tissues and maternal contamination.
             - This report must be accompanied by the corresponding karyotype image and must be interpreted within the clinical context of the patient and according to medical criteria.

Test Code: 5446

Karyotype, fibroblasts

Previous References Values

New References Values

Technique: Cell culture. G-Banded Chromosomal Analysis (2020). Resolution level: 400-550 bands. Minimum number of metaphases studied: 15.
Limitations: This result does not include the findings interpreted as variants of normality (European Cytogeneticists Association (ECA) http://www.eca.eu/EN/GUIDELINES.html) and is subject to the limitations of this type of study. such as, among others, the presence of certain mosaic situations, small chromosomal abnormalities, discrepancies between tissues and contamination with maternal cells.
This report must be accompanied by the image of the corresponding karyotype and must be interpreted within the clinical context of the patient and according to medical criteria.

4. Methodology
             Cell culture. Identification by G bands (ISCN 2020) from two independent cultures. Resolution: 400-550 bands. Minimum number of metaphases analyzed: 20.

5. Limitations and recommendations
             - The present result does not include the chromosomal findings interpreted as variants of normality (European Cytogeneticists Association (ECA) http://www.eca.eu/EN/GUIDELINES.html) and is subject to the limitations of this type of studies, such as, among others, the presence of certain situations in mosaic, chromosomal cryptic or small size anomalies, discrepancies between tissues and maternal contamination.
             - This report must be accompanied by the corresponding karyotype image and must be interpreted within the clinical context of the patient and according to medical criteria.

Test Code: 9605

Karyotype, culture

Previous References Values

New References Values

Technique: Cell culture for 48-72 hours stimulated with PHA (phytohemagglutinin). G-Banded Chromosomal Analysis (2020). Resolution level: 400-550 bands. Minimum number of metaphases studied: 15.
Limitations: This result does not include chromosomal findings interpreted as normal variants (European Cytogeneticists Association (ECA) http://www.eca.eu/EN/GUIDELINES.html) and is subject to the limitations of this type of analysis. studies, such as, among others, the presence of certain mosaic situations, small chromosomal abnormalities and discrepancies between tissues.

4. Methodology
             Cell culture (48h and 72h) stimulated with PHA (phytohemaglutinin). Identification by G bands (ISCN 2020). Resolution: 400-550 bands. Minimum number of metaphases analyzed: 15.

5. Limitations and recommendations
             - The present result does not include the chromosomal findings interpreted as variants of normality (European Cytogeneticists Association (ECA) http://www.eca.eu/EN/GUIDELINES.html) and is subject to the limitations of this type of studies, such as, among others, the presence of certain situations in mosaic, chromosomal cryptic or small size anomalies and discrepancies between tissues.
            - This report must be accompanied by the corresponding karyotype image and must be interpreted within the clinical context of the patient and according to medical criteria.

Test Code: 3474

Karyotype, chorionic villus (culture)

Previous References Values

New References Values

Technique: Cell culture. Analysis of two independent cultures. G-Banded Chromosomal Analysis (2020). Resolution level: 400-550 bands. Minimum number of metaphases studied: 20.
Limitations: This result does not include the findings interpreted as variants of normality (European Cytogeneticists Association (ECA) http://www.eca.eu/EN/GUIDELINES.html) and is subject to the limitations of this type of study. such as, among others, the presence of certain mosaic situations, small chromosomal abnormalities, discrepancies between tissues and contamination with maternal cells.
This report must be accompanied by the image of the corresponding karyotype and must be interpreted within the clinical context of the patient and according to medical criteria.

4. Methodology
             Cell culture. Identification by G bands (ISCN 2020) from two independent cultures. Resolution: 400-550 bands. Minimum number of metaphases analyzed: 20.

5. Limitations and recommendations
             - The present result does not include the chromosomal findings interpreted as variants of normality (European Cytogeneticists Association (ECA) http://www.eca.eu/EN/GUIDELINES.html) and is subject to the limitations of this type of studies, such as, among others, the presence of certain situations in mosaic, chromosomal cryptic or small size anomalies, discrepancies between tissues and maternal contamination.
             - This report must be accompanied by the corresponding karyotype image and must be interpreted within the clinical context of the patient and according to medical criteria.

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Thursday, March 16, 2023

Karyotype, total blood, amniotic fluid, haematological, tissue, total cord blood, fibroblasts, culture, chorionic villus (culture)

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Test Code: 684
Karyotype, total blood
Previous References Values	New References Values
Technique: Cell culture for 48-72 hours stimulated with PHA (phytohemagglutinin). G-Banded Chromosomal Analysis (2020). Resolution level: 400-550 bands. Minimum number of metaphases studied: 15. Limitations: This result does not include chromosomal findings interpreted as normal variants (European Cytogeneticists Association (ECA) http://www.eca.eu/EN/GUIDELINES.html) and is subject to the limitations of this type of analysis. studies, such as, among others, the presence of certain mosaic situations, small chromosomal abnormalities and discrepancies between tissues.	4. Methodology Cell culture (48h and 72h) stimulated with PHA (phytohemaglutinin). Identification by G bands (ISCN 2020). Resolution: 400-550 bands. Minimum number of metaphases analyzed: 15. 5. Limitations and recommendations - The present result does not include the chromosomal findings interpreted as variants of normality (European Cytogeneticists Association (ECA) http://www.eca.eu/EN/GUIDELINES.html) and is subject to the limitations of this type of studies, such as, among others, the presence of certain situations in mosaic, chromosomal cryptic or small size anomalies and discrepancies between tissues. - This report must be accompanied by the corresponding karyotype image and must be interpreted within the clinical context of the patient and according to medical criteria.
Test Code: 907
Karyotype, amniotic fluid
Previous References Values	New References Values
Technique: Cell culture. Analysis of two independent cultures. G-Banded Chromosomal Analysis (2020). Resolution level: 400-550 bands. Minimum number of metaphases studied: 20. Limitations: This result does not include the findings interpreted as variants of normality (European Cytogeneticists Association (ECA) http://www.eca.eu/EN/GUIDELINES.html) and is subject to the limitations of this type of study. such as, among others, the presence of certain mosaic situations, small chromosomal abnormalities, discrepancies between tissues and contamination with maternal cells. This report must be accompanied by the image of the corresponding karyotype and must be interpreted within the clinical context of the patient and according to medical criteria.	4. Methodology Cell culture. Identification by G bands (ISCN 2020) from two independent cultures. Resolution: 400-550 bands. Minimum number of metaphases analyzed: 20. 5. Limitations and recommendations - The present result does not include the chromosomal findings interpreted as variants of normality (European Cytogeneticists Association (ECA) http://www.eca.eu/EN/GUIDELINES.html) and is subject to the limitations of this type of studies, such as, among others, the presence of certain situations in mosaic, chromosomal cryptic or small size anomalies, discrepancies between tissues and maternal contamination. - This report must be accompanied by the corresponding karyotype image and must be interpreted within the clinical context of the patient and according to medical criteria.
Test Code: 1016
Karyotype haematological
Previous References Values	New References Values
Technique: Cell culture (24 hours without stimulation and/or 72 hours stimulated with TPA (tetradecanoylphorbol acetate) or PHA (phytohemagglutinin), depending on diagnostic orientation). G-Banded Chromosomal Analysis (2020). Resolution level: 400-550 bands. Minimum number of metaphases studied: 20. Limitations: This result does not include the findings interpreted as variants of normality (European Cytogeneticists Association (ECA) http://www.eca.eu/EN/GUIDELINES.html) and is subject to the limitations of this type of study. , mainly the absence of growth of pathological cells, the possible non-detection of some mosaics and small structural alterations. This report must be accompanied by the image of the corresponding karyotype and must be interpreted within the clinical context of the patient and according to medical criteria.	4. Methodology Cell culture, with or without stimulation with TPA (tetradecanoylphorbol acetate) or PHA (phytohemagglutinin), depending on diagnostic orientation. Identification by G bands (ISCN 2020) in two independent cultures. Resolution: 300-400 bands. Minimum number of metaphases analyzed: 20 1) Myeloproliferative neoplasia, Myelodysplastic syndrome, Acute leukemia, not indicated: direct cultures and 24 hours without mitogen. 2) Multiple myeloma: direct cultures and 72 hours without mitogen. 3)Chronic Lymphoproliferative Syndrome of B cells, Chronic Lymphoid Leukemia: direct cultures and 72 hours stimulated with TPA. 4) Chronic Lymphoproliferative Syndrome of T cells: direct cultures and 72 hours stipulated with PHA. 5. Limitations and recommendations - This result is issued following the criteria of the European Cytogeneticists Association (ECA),http//www.e-c-a.eu/EN/GUIDELINES.html). - This study does not include chromosomal findings interpreted as normal variants and is subject to the limitations of this type of study, mainly the absence of growth of pathological cells, the possible non-detection of some mosaics and small structural alterations. - The guidelines indicate that the conventional karyotype is not the most recommended analysis in cases of MM, although it can be performed to complete the study if there is a sufficient sample (Multiple Myeloma Guideline of the Spanish Group, 2021). - This report must be accompanied by the corresponding karyotype image(s) and must be interpreted within the clinical context of the patient and according to medical criteria.
Test Code: 2260
Karyotype, tissue
Previous References Values	New References Values
Technique: Cell culture. G-Banded Chromosomal Analysis (2020). Resolution level: 400-550 bands. Minimum number of metaphases studied: 15. Limitations: This result does not include the findings interpreted as variants of normality (European Cytogeneticists Association (ECA) http://www.eca.eu/EN/GUIDELINES.html) and is subject to the limitations of this type of study. such as, among others, the presence of certain mosaic situations, small chromosomal abnormalities, discrepancies between tissues and contamination with maternal cells. This report must be accompanied by the image of the corresponding karyotype and must be interpreted within the clinical context of the patient and according to medical criteria.	4. Methodology Cell culture. Identification by G bands (ISCN 2020). Resolution: 400-550 bands. Minimum number of metaphases analyzed: 15. 5. Limitations and recommendations - The present result does not include the chromosomal findings interpreted as variants of normality (European Cytogeneticists Association (ECA) http://www.eca.eu/EN/GUIDELINES.html) and is subject to the limitations of this type of studies, such as, among others, the presence of certain situations in mosaic, chromosomal cryptic or small size anomalies, discrepancies between tissues and maternal contamination. - This report must be accompanied by the corresponding karyotype image(s) and must be interpreted within the clinical context of the patient and according to medical criteria.
Test Code: 2533
Karyotype, total cord blood
Previous References Values	New References Values
Technique: Cell culture for 48-72 hours stimulated with PHA (phytohemagglutinin). G-Banded Chromosomal Analysis (2020). Resolution level: 400-550 bands. Minimum number of metaphases studied: 15. Limitations: This result does not include chromosomal findings interpreted as normal variants (European Cytogeneticists Association (ECA) http://www.eca.eu/EN/GUIDELINES.html) and is subject to the limitations of this type of analysis. studies, such as, among others, the presence of certain mosaic situations, small chromosomal abnormalities and discrepancies between tissues. This report must be accompanied by the image of the corresponding karyotype and must be interpreted within the clinical context of the patient and according to medical criteria.	4. Methodology Cell culture (48h and 72h) stimulated with PHA (phytohemaglutinin). Identification by G bands (ISCN 2020). Resolution: 400-550 bands. Minimum number of metaphases analyzed: 15. 5. Limitations and recommendations - The present result does not include the chromosomal findings interpreted as variants of normality (European Cytogeneticists Association (ECA) http://www.eca.eu/EN/GUIDELINES.html) and is subject to the limitations of this type of studies, such as, among others, the presence of certain situations in mosaic, chromosomal cryptic or small size anomalies, discrepancies between tissues and maternal contamination. - This report must be accompanied by the corresponding karyotype image and must be interpreted within the clinical context of the patient and according to medical criteria.
Test Code: 5446
Karyotype, fibroblasts
Previous References Values	New References Values
Technique: Cell culture. G-Banded Chromosomal Analysis (2020). Resolution level: 400-550 bands. Minimum number of metaphases studied: 15. Limitations: This result does not include the findings interpreted as variants of normality (European Cytogeneticists Association (ECA) http://www.eca.eu/EN/GUIDELINES.html) and is subject to the limitations of this type of study. such as, among others, the presence of certain mosaic situations, small chromosomal abnormalities, discrepancies between tissues and contamination with maternal cells. This report must be accompanied by the image of the corresponding karyotype and must be interpreted within the clinical context of the patient and according to medical criteria.	4. Methodology Cell culture. Identification by G bands (ISCN 2020) from two independent cultures. Resolution: 400-550 bands. Minimum number of metaphases analyzed: 20. 5. Limitations and recommendations - The present result does not include the chromosomal findings interpreted as variants of normality (European Cytogeneticists Association (ECA) http://www.eca.eu/EN/GUIDELINES.html) and is subject to the limitations of this type of studies, such as, among others, the presence of certain situations in mosaic, chromosomal cryptic or small size anomalies, discrepancies between tissues and maternal contamination. - This report must be accompanied by the corresponding karyotype image and must be interpreted within the clinical context of the patient and according to medical criteria.
Test Code: 9605
Karyotype, culture
Previous References Values	New References Values
Technique: Cell culture for 48-72 hours stimulated with PHA (phytohemagglutinin). G-Banded Chromosomal Analysis (2020). Resolution level: 400-550 bands. Minimum number of metaphases studied: 15. Limitations: This result does not include chromosomal findings interpreted as normal variants (European Cytogeneticists Association (ECA) http://www.eca.eu/EN/GUIDELINES.html) and is subject to the limitations of this type of analysis. studies, such as, among others, the presence of certain mosaic situations, small chromosomal abnormalities and discrepancies between tissues.	4. Methodology Cell culture (48h and 72h) stimulated with PHA (phytohemaglutinin). Identification by G bands (ISCN 2020). Resolution: 400-550 bands. Minimum number of metaphases analyzed: 15. 5. Limitations and recommendations - The present result does not include the chromosomal findings interpreted as variants of normality (European Cytogeneticists Association (ECA) http://www.eca.eu/EN/GUIDELINES.html) and is subject to the limitations of this type of studies, such as, among others, the presence of certain situations in mosaic, chromosomal cryptic or small size anomalies and discrepancies between tissues. - This report must be accompanied by the corresponding karyotype image and must be interpreted within the clinical context of the patient and according to medical criteria.
Test Code: 3474
Karyotype, chorionic villus (culture)
Previous References Values	New References Values
Technique: Cell culture. Analysis of two independent cultures. G-Banded Chromosomal Analysis (2020). Resolution level: 400-550 bands. Minimum number of metaphases studied: 20. Limitations: This result does not include the findings interpreted as variants of normality (European Cytogeneticists Association (ECA) http://www.eca.eu/EN/GUIDELINES.html) and is subject to the limitations of this type of study. such as, among others, the presence of certain mosaic situations, small chromosomal abnormalities, discrepancies between tissues and contamination with maternal cells. This report must be accompanied by the image of the corresponding karyotype and must be interpreted within the clinical context of the patient and according to medical criteria.	4. Methodology Cell culture. Identification by G bands (ISCN 2020) from two independent cultures. Resolution: 400-550 bands. Minimum number of metaphases analyzed: 20. 5. Limitations and recommendations - The present result does not include the chromosomal findings interpreted as variants of normality (European Cytogeneticists Association (ECA) http://www.eca.eu/EN/GUIDELINES.html) and is subject to the limitations of this type of studies, such as, among others, the presence of certain situations in mosaic, chromosomal cryptic or small size anomalies, discrepancies between tissues and maternal contamination. - This report must be accompanied by the corresponding karyotype image and must be interpreted within the clinical context of the patient and according to medical criteria.